Abstract: Service quality is a significant metric for gauging the achievement of seaports around the world. This study focused on evaluating and comparing the service quality of major seaports in Southern and Eastern Africa. 347 structured questionnaires were distributed to seaport customers who were the study respondents for data collection purpose. SERVQUAL model based on Responsiveness, Reliability, Tangibility, Assurance and Empathy measures were used to compute and compare the service quality of six selected seaports from Southern and Eastern Africa.