Abstract: The present paper reports the regeneration potential and distribution pattern of natural forest in Taknaur Reserve Forest of Uttarkashi forest division of Garhwal Himalaya, Uttarakhand. A total of 29 tree species were recorded from the area. Density and distribution of seedlings, saplings and trees were assessed along the gradient of altitude using the quadrat size 1x1m (for seedlings) 5x5m (for saplings) and 10x10m (for trees). The numbers of individuals, frequency were recorded and height of the species in the quadrat at 100m drops in altitude. Seedling densities varied markedly among the species and altitude. Lower elevation was dominated with Pinus roxburghii and Quercus leucotrichophora was found co-dominant species whereas, Buxus wallichiana and Quercus floribunda was dominant and Taxus baccata was co-dominant species in site two (1900-2500m asl). While upper elevations were dominated by Buxus wallichiana, Quercus floribunda and Abies pindrow, Cedrus deodara, respectively, for site 3rd and 4th . However, the highest (10000 ha-1) seedling density , was reported for Pinus roxburghii at site 1 followed by Cedrus deodara (9000 individuals ha-1 ) at site 2, Buxus wallichiana, Taxus buccata, Quercus floribunda Abies pindrow and Cedrus deodara (4000 individual ha-1 of each species) at site 3 and 4, respectively. It is remarkable that economically and ecologically valued species Acer accuminatum, Ulmus wallichiana, Betula utilis Acer oblongum which are already in vulnerable in their status were found absent in seedling stage. These species were also reported as regularly distributed in tree and sapling stratum, which is very alarming for the conservation point of view. In tree layer the most of the species (65.16%) were distributed contagiously and remaing (34.84%) were distributed randomly.