Abstract: Reactive oxygen species (ROS) play a pivotal role in the development of oxidative stress-related health disorders, driving the ongoing pursuit of natural antioxidants as potential remedies. Desmodium gangeticum (DG), a medicinal plant recognized for its antioxidant properties, The plants roots, shoots, leaves, and bark have all exhibited medicinal attributes, notably its capacity to counteract oxidative stress which is an intricate process often initiated by iron-induced Fenton reactions that generate harmful free radicals. Our study delves into the multifaceted role of DG, encompassing its effects on cell viability, ROS reduction, and its potential as an iron chelator Given DGs rich content of flavonoids and polyphenols, we hypothesized that these compounds could interact with iron, potentially preventing its participation in free radical generation.