Abstract: The results were obtained from the investigation shows that the individual application of nitrogen and phosphorus and their interaction significantly influence the physico- chemical properties of guava cv. L-49. Treatments with higher doses of nitrogen were found very effective in improve the physico- chemical parameters of guava fruit. The maximum fruit diameter (7.40 cm), fruit length (7.31 cm) and pulp weight (200.01g) was recorded by combined application of 600 g N and 400 g P2O5 (N2P2) per tree. The effect of treatment interaction was non significant on the TSS, acidity and tss/acidity ratio. The maximum total sugars (7.987%) were recorded in N1P2, non-reducing sugar (4.097%) were recorded in N2P0 and maximum reducing sugar (4.253%) was recorded in N1P0 treatment combination.