Abstract: Manipur, a rich state of fauna and flora and their product are used as medicine from the immorable time. People of Manipur use large number of animal species in zoo-therapy treatment for different ailments. Both domestic and wild animals are made as whole or different parts for treatment of almost all the commonly occurring health problems. Health care practice includes local and oral applications to cure diseases. People have relationship with animals at culture, spiritual and materials levels. The present paper attempt to document the traditional ethno-zoological knowledge of our state. Altogether thirty three species of animals and their product, nature of ailment and mode of treatment has been presented. Five species of invertebrate and twenty eight species of vertebrate was listed. In invertebrates five species belonging 5 families and in vertebrates twenty eight species belong to 20 families. Among vertebrates 3 species are endangered, 4 species are vulnerable, 4 species are near threatened and the rest are least concern expect human. These animals were used in treatment of 35 ailments as traditional medicines for health care.