Abstract: Background: Lassa fever is a severe viral hemorrhagic disease, primarily prevalent in West Africa, and is transmitted when individuals come into contact with food contaminated by urine or feces from rodents, particularly rats. The key goal of Behavioral Change Communication (BBC) is to enhance knowledge and attitudes among individuals, families, communities, and health workers, ultimately promoting behavioral changes that can effectively prevent the spread of the disease. Therefore, this study aims to assess the effect of health education intervention on the knowledge and attitude towards prevention practices among nursing mothers in Akinyele, Ibadan, Oyo State, Nigeria.