Abstract: A research was conducted at Gokuleshwor VDC of Darchula district during 2015 to determine the performance of different substrate for the production of Oyster The research was carried out by growing florida species of Pleurotus (Oyster Mushroom), on five different substrates namely banana leaves, rice straw, wheat straw, mixture of rice and wheat straw and saw dust under one factor CRD design with four replication during summer with an average daily temperature of 260C- 300C and RH ranging from 80 to 100%. Among the five different treatments banana leaves was found to be suitable as it obtained more total yield per five kg of substrate i. e. 4.76 kg followed by other substrates.